Sperm Proteomics Reveals Intensified Selection on Mouse Sperm Membrane and Acrosome Genes
نویسندگان
چکیده
منابع مشابه
Inhibition of mouse acrosome reaction and sperm-zona pellucida binding by anti-human sperm membrane protein 1 antibody.
AIM To investigate the possible functions of human sperm membrane protein (hSMP-1) in the process of fertilization. METHODS A 576-bp cDNA fragment of HSD-1 gene coding for the extracellular domain of hSMP-1 was cloned and expressed. The localization of this protein on human and mouse sperm was determined by indirect immunofluorescent staining by using anti-recombinant hSMP-1 (anti-rhSMP-1) an...
متن کاملTransgenic mouse sperm that have green acrosome and red mitochondria allow visualization of sperm and their acrosome reaction in vivo.
In the present paper, we introduce a transgenic mouse line whose sperm express green fluorescent protein (GFP) in their acrosome and red fluorescent protein (RFP) in their mitochondria [B6D2F1- Tg(CAG/su9-DsRed2, Acr3-EGFP)RBGS002Osb]. The dual fluorescent sperm showed normal fertilizing ability in both in vivo and in vitro fertilization and the sperm could be observed through uterine and ovidu...
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October 2005 | Volume 3 | Issue 10 | e347 | e353 Viewed under a microscope, the coronavirus appears almost beautiful, thanks to the halo-like crown formed by its surface proteins. (“Corona” means “crown” in Latin.) Aesthetics aside, this genus of viruses is responsible for a wide range of animal and human diseases, from the common cold to the deadly severe acute respiratory syndrome, familiarly...
متن کاملRedistribution of mouse sperm surface galactosyltransferase after the acrosome reaction
Gamete recognition in the mouse is mediated by galactosyltransferase (GalTase) on the sperm surface, which binds to its appropriate glycoside substrate in the egg zona pellucida (Lopez, L. C., E. M. Bayna, D. Litoff, N. L. Shaper, J. H. Shaper, and B. D. Shur, 1985, J. Cell Biol., 101:1501-1510). GalTase has been localized by indirect immunofluorescence to the dorsal surface of the anterior spe...
متن کاملEffect of swim-up sperm washing and subsequent capacitation on acrosome status and functional membrane integrity of normal sperm.
OBJECTIVE Sperm preparation techniques select sperm population with improved sperm motion characteristics. We sought to determine whether the swim-up technique selects spermatozoa with the ability to undergo hypoosmotic swelling, and how swim-up and subsequent capacitation affect the acrosome reaction rate. METHODS Semen specimens from 15 normal donors were divided into unprocessed, swim-up, ...
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ژورنال
عنوان ژورنال: Molecular Biology and Evolution
سال: 2010
ISSN: 1537-1719,0737-4038
DOI: 10.1093/molbev/msq007